Chronological Lab Notebook

Pushing copper into sunstones, this test 1100C for 48h, needs more for longer!

Cleared out dozens of tubs and a bunch of petri dishes with duckweed experiments. Sadly ruby batch got water mold, washed a few in bleach + water to attempt rex. Ditto miraculin bunch.
Anything GMO got bleached to death.
Yay fresh start.

Beautiful blues (other colors too with angle change) thanks to thin film effects in in this biofilm

Rough test, looks like 30-40mg per bottle (guesstimate)

Media: 25 ml MS media with 25ul bap, 2.5ul NAA, 50ul timentin, 200ul PPM

PPM only included second time after first got mold. (Pic shows mold) (Todo other pics)

Agro dip for 1h then 24h co-culture on plain ms media, then rinse in dilute bleach (2nd time, mold inspired), then tfer to antibiotic media. Here's hoping

I found this old product at SCRAPS PDX. Its a stack: PET carrier, some sort of release layer, shiny blue + aluminium layer and some heat activated adhesive. OG use was getting selectively stuck to laser printer toner to give your printed pages some bling. WIth a little heat it sticks to PLA quite nicely, although even surface contact seems important.

So at least some seem to be making stable daughters :)
GOsh I have so much stuff I should document and make notes of but I might have to declare bankruptcy on some of them, I'm behind and need to do more new experiments.

My poor duckweed stock; I cleaned em out and then forgot to add nutrients (I think)? Just noticed the state of them. Added some quickly, more than usual, will see what happens.

Hacked a UV camera to work on my mac!
https://github.com/johnowhitaker/uvcam
https://www.amazon.com/dp/B0H15QWXWB

Last transform was mid-april, most of these are from older ones. I collected some red fronds together to show off, here's the collection. They don't thrive, but I think there are some new small ones? Any left along end up surrounded by green though. Got some new tips for transform to try.

Lemna major vs minor (I guess major is now Spirodela polyrhiza but still)

Separating out some algae (also starting to think about ordering some chlorella and spirulina for edible algae experiments). Sorting old duckweed, about to sort new and big duckweed from whitaker ponds slough. Some water mold on one old dish. Some glow in eyGFPuv dwe but mostly from damage, TBD what grows.

After switching from tween 80 to span 80 (lol) I can finally do water in oil droplets, even with this crummy test droplet generator. (5% span 80 in mineral oil for this test). Todo wider channels for the oil since it is more viscous. See X for video, mesmerizing

Droplet gen working, Todo note on tween 80 vs span 80,

Indoor plant yielded a nice purple tomato, picked a little early, seeds preserved and packagred to share. Remaining indoor plants went out to join the flourishign outdoor ones now that some seeds are in the ba nk.
Some seeds on tissue, some left in water for two days then dried on coffee filter.

Mollala river, first try panning

Lots more crystal fun documented on johnoslab, including fringes caused by blowing over the cooling+evaporating slide to change the rate of crystal growth

I heated a pellet of polycaprolactone (soft thermoplastic) and stuck it to my 3D printer hotend with tweezers.
I then held a small industrial diamond (there were plenty of loose ones in a pack of rock carving bits I bough recently) beneeath it and pressed it up into the still-soft plastic.
Then I had codex whip up a UI to control the printer via the octoprint API.
I can now scribe tiny patterns into a glass microscope slide, to steer crystal growth. First tests cooling + evaporating now :)