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Duckweed tfm

Tuesday, March 3, 2026

Notebook Page

Notebook page for 2026-03-03

Experiment Gallery

Theraflu under PLM
Theraflu under PLM
syringe slam can be harsh, but some red indicates happy (under 365nm)
syringe slam can be harsh, but some red indicates happy (under 365nm)
Compare to those that just sat in agro mix for a bit
Compare to those that just sat in agro mix for a bit
I dream of a cocktail with modded duckweed that actually tastes like something.
I dream of a cocktail with modded duckweed that actually tastes like something.

Daily Notes

Attempting tissue-culture-free agro transform of duckweed (lemna minor). I ran a few diff exps this weekend, and more excitingly noticed a red frond in some that I'd tried to tfm on Feb 9th, hoping that means the meristem took in the genes and this is a properly transgenic new frond. Rough protocol:
- A loopful of agro into infiltration mix, in a syringe with some duckweed fronds
- Pull back plunger while blocking end, and release. The sudden vacuum+slam (in my head at least) helps push agro into the interstices of the fronds.
Will share more depending on how more recent tests go.