Experiment Gallery
Daily Notes
New run:
- Pseudomonas in liquid culture for ~18h
- Spun down, removed supernatant, add 10% gly (in 1.5ml tube)
- Spun down, removed supernatant, add 10% gly
- Spun down, removed supernatant, add 30uL of 10% gly (pellet by this stage was pretty small given my weak 'fuge)
- Added 3uL DNA (from a mini-prep I did yday, aeBLUE on a kanamycin resistant backbone)
- Did this in two tubes. One got 1.2mm gap, one got 2.4mm. 10 zaps each. On the 2.4mm gap, peak was ~5V (~10kV/cm). On the 1.2mm gap I didn't get a clear voltage reading, not sure what went wrong.
- 1h in 200uL LB broth @30C to recover before plating 100uL on Kan plates. Now we wait. Only worry: cells might have been stationary phase, might try a repeat with fresh culture in a few hours.