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Orange environmental yeast, green attempt #3

Wednesday, April 8, 2026

Experiment Gallery

A few small colonies, likely only form where density was high enough that they could overcome the antibiotic pressure
A few small colonies, likely only form where density was high enough that they could overcome the antibiotic pressure
Orange yeast, to add to my collection
Orange yeast, to add to my collection

Daily Notes

I've isolated a nice orange yeast that was growing on a neglected old plate.
I received my third plasmid design, another attempt at green. I streaked some out from the glycerol stock they provided yesterday, but this morning noticed only a few small colonies at the start of the streak... and then realized: I had forgotten to change the vector from the default (pUC57 with ampicillin resistance) - not the kan-resistant vector I wanted! Ah well. I have some old AMP plates in the fridge from last year, which I've streaked it out on, and if those are too old I'll make up some more with my last few uL of AMP solution. And then think about subcloning into a kan vector to match the other colors perhaps? Or just make art on plain LB and rely on them all not losing color too fast.