Experiment Gallery
Daily Notes
Well, I tried growing out the e.c. with my biliverdin plasmid. No obvious color in the colonies (maybe a little more yellowish than normal?) but the agar over time has shifted color. I think as I feared the biliverdin is diffusing out, and it's more bruise-brown than obvious green in the LB agar. Might need to try operation treefrog.
Also, this is growing out the cloning strain I got from genscript. The plasmid DNA tubes they sent looked empty, with the dry ice I suspect the DNA was freeze-dried in the (no parafilm) tubes. I added sterile water, and tried out my spec method to see if DNA was present, seems like probably? I did a transform but got one (1) colony so something funky going on. Next trying liquid culture of the stuff I already have, and leaving things for longer to see what happens.