Ln Lab Notes
Back to timeline

Rg miniprep

Wednesday, March 11, 2026

Experiment Gallery

Collection grows
Collection grows
DNA stain + 2ul of the miniprep result, but of glow?
DNA stain + 2ul of the miniprep result, but of glow?
Experiment photo
First plate from the stab, picked a glowy colony (gfp in bacteria is marker for this plasmid)
First plate from the stab, picked a glowy colony (gfp in bacteria is marker for this plasmid)

Daily Notes

Miniprep on pCambria RubyGreen, think I got some DNA? Todo machine to quantify amt. Also planted plants a few days ago.
My 'fuge is weak so in the miniprep I use a syringe filter rather than spinning down after mixing all the things, and just give it extra time when sending stuff through the column. Also for this one I just scraped a big loopful of cells from the plate into A1 rather than growing out liquid culture